Product Description

C1q/Factor D-depleted serum is normal human serum in which complement proteins C1q and factor D have been depleted by ion exchange and immunoaffinity chromatography. The product is tested for C1q and factor D proteins by double immunodiffusion and the absence of C1q and factor D is measured by testing classical and alternative pathway function. The product is certified to exhibit less than 5% classical and alternative pathway activity. After reconstitution with purified C1q protein (70 µg/mL), C1q/Factor D-depleted serum is certified to possess a functional classical pathway for complement activation (Morgan, B.P. (2000); Dodds, A.W. and Sim, R.B. (1997)).Similarly, a functional alternative pathway can be reconstituted by addition of purified factor D (1.4 µg/mL) indicating that all other complement components necessary for alternative pathway activation are present. Lectin pathway activity is not tested, but it would be expected to be active.

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