Product Description

SGVB⁺⁺ is a specialized buffer which is low in ionic strength (due to the low NaCl concentration), but isotonic (due to the sucrose).This combination of properties allows complement assays or binding assays to be performed with cells at low ionic strength, which improves binding, while the sucrose keeps the cells from lysing due to the high concentration of sucrose.This buffer is practically identical to DGVB buffer except that they use D-glucose instead of sucrose to maintain their isotonic characteristic.These buffers are isotonic with VBS, GVB, PBS, etc. meaning that the osmotic pressure on cell membranes will be the same even though the salt concentration is low.SGVB⁺⁺ buffer is especially useful in C1 assays (Dodds, A.W. and Sim, R.B. (1997)) and in binding assays such as those measuring the binding of factor B or factor H to EsC3b cells (sheep erythrocytes bearing surface-bound C3b) (Pangburn, M.K. and Muller-Eberhard, H.J. (1978); Pangburn, M.K., et al. (1980)).These interactions are sensitive to the salt concentration and lowering the ionic strength can enhance binding 5- to 10-fold.

(For full product description see link...)

Complement Technology，Inc。（CompTech）致力于为生物医学研究界提供最高质量的补充试剂。CompTech的业主和员工在补充领域拥有超过80年的综合经验。我们是我们销售的补充试剂的原始制造商，我们努力成为最优质试剂的最低成本供应商。我们不断扩大我们生产的试剂系列，以满足不断变化的研究需求。我们欢迎您提供所需新试剂的建议。