Product Description

C4-Dpl is normal human serum in which C4 has been removed by immunoaffinity chromatography. The product is tested for the absence of C4 by functional assays for classical and alternative pathway activity and for C4 protein by double immunodiffusion. C4-Dpl is certified to possess a functional alternative pathway for complement activation (Morgan, B.P. (2000); Dodds, A.W. and Sim, R.B. (1997)).Similarly, a functional classical pathway can be reconstituted by addition of purified C4 protein (0.40 mg/mL) indicating that all other complement components necessary for classical and alternative pathway activation are present.The absence of C4 also prevents complement activation by the lectin pathway, but the function of this pathway is not tested.

Note that this C4-Dpl serum does not contain C4-derived antigens because it has been depleted by immunoaffinity adsorption of C4.

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